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The BBC Scotland debate is being held in the spectacular setting of Hopetoun House, on the outskirts of Edinburgh.
It will feature SNP leader Nicola Sturgeon, Labour's Kezia Dugdale and Ruth Davidson of the Conservatives.
Liberal Democrat leader Willie Rennie and Patrick Harvie of the Scottish Greens are also on the panel.
The hour-long discussion, which is being broadcast live on BBC1 Scotland and the BBC news website between 20:00 and 21:00, will be chaired by the BBC's Sarah Smith.
Questions will be put to the politicians by an audience of voters on topics including independence, jobs, health and education.
It will be the third and final televised leaders' debate before the election, with BBC Scotland and STV having held one each earlier in the campaign.
The campaign has so far been largely been dominated by the question of how best to use new powers over tax - particularly income tax - and welfare that will be devolved to the Scottish Parliament next year.
The parties have also focused heavily on the future of Scotland's fully-devolved education and health systems.
And the issue of Scottish independence has never been too far from the surface - with Ms Sturgeon saying in a interview with the Sunday Herald newspaper ahead of the debate that she was confident that a second referendum would be held while she is first minister.
Opinion polls have suggested the SNP is on course to win a second successive majority - and third successive term in government - with Labour and the Conservatives in a battle for second.
They have also suggested the Scottish Greens could challenge the Liberal Democrats for fourth place.